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Determination of illicit and medicinal drugs and their metabolites in oral fluid and preserved oral fluid by liquid chromatography-tandem mass spectrometry.

Concheiro M, de Castro A, Quintela O, Cruz A, López-Rivadulla M

Forensic Toxicology Service. Institute of Legal Medicine, University of Santiago de Compostela Spain, C/ San Francisco s/n, 15782, Santiago de Compostela, Spain.

An LC-MS/MS method using 0.5 ml of oral fluid was developed for the determination of morphine, codeine, 6-monoacetylmorphine, methadone, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, benzoylecgonine, cocaine, delta-9-tetrahydrocannabinol, zolpidem, zopiclone, alprazolam, clonazepam, oxazepam, nordiazepam, lorazepam, flunitrazepam, diazepam, diphenhydramine and amitriptyline. The method was fully validated in terms of linearity (the method was linear between 1-5 microg/L and 100-200 microg/L) recoveries (7.5-82.6%), within-day and between-day precisions and accuracies (CV and MRE, both <15%), limits of detection (0.5 microg/L) and quantitation (the lowest point on the calibration curve), relative ion intensities, freeze-and-thaw stability and matrix effect. The method was applied to preserved oral fluid collected by a special commercial device, the StatSure Saliva Sampler.

Published 27 June 2008 in Anal Bioanal Chem, 391(6): 2329-38.
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