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High-performance liquid chromatography accelerator mass spectrometry: correcting for losses during analysis by internal standardization.

Lappin G, Simpson M, Shishikura Y, Garner C

Xceleron Ltd, The Biocentre, Innovation Way, York YO10 5NY, UK. graham.lappin@xceleron.com

A method was developed to account for analytical losses of (14)C-analyte when determining the concentration in biological samples using chromatographic separation and analysis by accelerator mass spectrometry. From the equations of J. Vogel and A.H. Love (in: A.L. Burlingame (Ed.), Methods in Enzymology, Academic Press, New York, 2005), new equations were derived to describe the isotopic dilution of a chromatographically isolated (14)C-analyte. The analytical recovery for each sample was determined by the use of the UV response for nonlabeled analyte, as an internal standard against a standard curve. The slope of the curve was substituted into the equations to provide a method of accurately determining the analyte concentration.

Published 26 May 2008 in Anal Biochem, 378(1): 93-5.
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