Chromatography Research Today is a free monthly online journal that collates and summarizes the latest research about Chromatography, including details on column chromatography, gas chromatography (gc), liquid chromatograpy, hplc.

Comparison of capillary electrophoresis and high performance liquid chromatography for determination of flavonoids in Achillea millefolium.

Kocevar N, Glavac I, Injac R, Kreft S

University of Ljubljana, Faculty of Pharmacy, Department of Pharmaceutical Biology, Askerceva 7, 1000 Ljubljana, Slovenia. nina.kocevar@ffa.uni-j.si

Flavonoids represent an important bioactive component in Achillea millefolium. The comparison of the most commonly used analytical methods for the identification and quantification of flavonoids, capillary electrophoresis (CE) and high performance liquid chromatography (HPLC), is presented. The methods were optimized and validated. Using a 20 mM borate buffer with 30% (v/v) of methanol (pH 9.3) in the CE analysis and a gradient elution with water-acetonitrile mobile phase in the HPLC analysis, sufficient separation of the analytes was achieved. A relatively high injection volume in the CE analysis (30 mbar x 30s) enabled low limit of detection (LOD) (0.3-0.7 mg/L). Repeatability of both methods was acceptable (relative standard deviation of peak area were <6%). Additionally, the amount of flavonoids in a real sample of the dried herbal drug was determined.

Published 14 January 2008 in J Pharm Biomed Anal, 46(3): 609-14.
Full-text of this article is available online (may require subscription).

© 2005-2008 Chromatography Research Today. All Rights Reserved.