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Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry.

Poirier L, Amiard JC, Mondeguer F, Quiniou F, Ruiz N, Pouchus YF, Montagu M

Université de Nantes, Nantes Atlantique Universités, SMAB, EA2160, Faculté de pharmacie, 1 rue G. Veil-BP 53508, Nantes F-44000, France. laurence.poirier@univ-nantes.fr

Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found.

Published 30 July 2007 in J Chromatogr A, 1160(1): 106-13.
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