Chromatography Research Today is a free monthly online journal that collates and summarizes the latest research about Chromatography, including details on column chromatography, gas chromatography (gc), liquid chromatograpy, hplc.

Evaluation of atmospheric pressure ionization interfaces for quantitative measurement of sulfonamides in honey using isotope dilution liquid chromatography coupled with tandem mass spectrometry techniques.

Mohamed R, Hammel YA, LeBreton MH, Tabet JC, Jullien L, Guy PA

Nestlé Research Center, Nestec Ltd., P.O. Box 44, Vers-chez-les Blanc, 1000 Lausanne 26, Switzerland.

A comparison was made between electrospray, atmospheric pressure chemical and atmospheric pressure photospray ionizations to evaluate the MS/MS responses of standard sulfonamides and honey spiked samples. The sample preparation entails an acidic hydrolysis followed by a liquid/liquid extraction. Full method validation was realised by LC-APPI-MS/MS. Decision limit and detection capability were calculated for each analyte (at 50 microg/kg) and ranged between 53.6 and 56.9 and 57.5 and 63.2 microg/kg, respectively. Limits of detection and of quantification ranged, respectively, at 0.4-4.5 and 1.2-15.0 microg/kg. Precursor ion scan experiments of m/z 92 were also carried out as a survey experiment, linked with an enhanced product ion scan experiment to potentially identified additional sulfonamides via a library search.

Published 30 July 2007 in J Chromatogr A, 1160(1): 194-205.
Full-text of this article is available online (may require subscription).

© 2005-2008 Chromatography Research Today. All Rights Reserved.