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Counter-current chromatography separation scaled up from an analytical column to a production column.

Wood P, Ignatova S, Janaway L, Keay D, Hawes D, Garrard I, Sutherland IA

Brunel Institute for Bioengineering, Brunel University, Uxbridge UB8 3PH, UK. philip.wood@brunel.ac.uk

An analytical separation was performed on an analytical J-type counter-current chromatography (CCC) instrument using a 5.4 ml column, with a 1 ml/min mobile phase flow rate. This separation had a resolution of 0.69 and was achieved in 10 min. The same separation was performed using two 2300 ml columns connected in series at a flow rate of 850 ml/min using a production scale J-type centrifuge. This production scale separation was also obtained in 10 min with a resolution of 0.71. This represents an 850 times increase in productivity. This paper presents these separations and the underlying scale up theory.

Published 9 May 2007 in J Chromatogr A, 1151(1): 25-30.
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