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Application of high-speed counter-current chromatography for the isolation of 9'-cis-neoxanthin from fresh spinach.

Baldermann S, Reinhard A, Köhler N, Fleischmann P

Institute of Food Chemistry, Technical University of Braunschweig, Schleinitzstrasse 20, 38106 Braunschweig, Germany. s.baldermann@tu-bs.de

Preparative HSCCC (high-speed counter-current chromatography) could be applied for the isolation of 9'-cis-neoxanthin from a crude carotenoid extract of fresh spinach leaves. The separation was performed on a Pharma-Tech Research Corp. CCC 1000 with a solvent system composed of hexane:ethanol:water at a volume ratio of 5:5:4.5 at a flow rate of 3 mL/min and at 850 rpm, using the lower phase as mobile phase. 9'-cis-neoxanthin with a purity of up to 94% could be obtained with a single HSCCC purification step of the crude carotenoid extract.

Published 9 May 2007 in J Chromatogr A, 1151(1): 183-6.
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