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Isolation of high-purity casticin from Artemisia annua L. by high-speed counter-current chromatography.

Han X, Ma X, Zhang T, Zhang Y, Liu Q, Ito Y

Chinese Key Laboratory on National Reference Materials for Natural Products, Beijing UE Biotech Co. Ltd., Beijing 100035, China. ssisyhan@yahoo.com.cn

Following an initial clean-up step on silica, high-speed counter-current chromatography (HSCCC) was used to purify a flavone, casticin (5,3'-dihydroxy-3,6,7, 4'-tetramethoxyflavone), from an extract of the dried leaves of Artemisia annua L. The two-phase solvent system used was composed of n-hexane-ethyl acetate-methanol-water at an optimized volume ratio of 7:10:7:10 (v/v). HSCCC separation of 226.4 mg of crude sample (containing casticin at 16.5% purity after silica gel clean-up) yielded 36.3 mg of casticin with a purity of over 99% and 96.2% recovery. Identification of the target compound was performed by (1)H NMR, (13)C NMR, two-dimensional NMR, electrospray ionization MS, IR and UV.

Published 9 May 2007 in J Chromatogr A, 1151(1): 180-2.
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