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Affinity chromatography: a useful tool in proteomics studies.

Azarkan M, Huet J, Baeyens-Volant D, Looze Y, Vandenbussche G

Laboratoire de Chimie Générale (CP: 609), Faculté de Médecine, Université Libre de Bruxelles, Campus Erasme, 808, route de Lennik, B-1070 Bruxelles, Belgium.

Separation or fractionation of a biological sample in order to reduce its complexity is often a prerequisite to qualitative or quantitative proteomic approaches. Affinity chromatography is an efficient protein separation method based on the interaction between target proteins and specific immobilized ligands. The large range of available ligands allows to separate a complex biological extract in different protein classes or to isolate the low abundance species such as post-translationally modified proteins. This method plays an essential role in the isolation of protein complexes and in the identification of protein-protein interaction networks. Affinity chromatography is also required for quantification of protein expression by using isotope-coded affinity tags.

Published 10 April 2007 in J Chromatogr B Analyt Technol Biomed Life Sci, 849(1): 81-90.
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