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Direct detection method of oligosaccharides by high-performance liquid chromatography with charged aerosol detection.

Inagaki S, Min JZ, Toyo'oka T

Division of Bio-Analytical Chemistry, School of Pharmaceutical Sciences, COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Surugaku, Shizuoka 422-8526, Japan.

A simple and rapid detection method of oligosaccharides using high-performance liquid chromatography with a charged aerosol detection (HPLC-CAD) was studied. The direct detection of a sialylglycopeptide (SGP) derived from egg yolk was accomplished by HPLC-CAD using an amido-silica column, and its limit of detection was 0.40 pmol [signal-to-noise ratio (S/N) = 3]. The sensitivity of this method was lower than that of the fluorescence detection; however, the method showed approximately 5 times higher sensitivity than that using the conventional UV absorbance detection. Furthermore, this method was used for the analysis of the acid hydrolysis products of SGP. Monosialo- and asialo-oligosaccharides as well as free sialic acid were detected without using fluorescent derivatization. These results indicate that the present method is a new tool for the analysis of oligosaccharides.

Published 2 April 2007 in Biomed Chromatogr, 21(4): 338-42.
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