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Metal-binding molecules in the organs of Mus musculus by size-exclusion chromatography coupled with UV spectroscopy and ICP-MS.

González-Fernández M, García-Barrera T, Arias-Borrego A, Bonilla-Valverde D, López-Barea J, Pueyo C, Gómez-Ariza JL

Departamento de Química y Ciencia de los Materiales, Facultad de Ciencias Experimentales, Campus de El Carmen, Avda. de Fuerzas Armadas S/N, 21007, Huelva, Spain.

Mus musculus mice have been investigated for the total elements content in different organs (lung, liver, spleen, kidney, brain, testicle, heart and muscle) and molecular mass distribution patterns of Mn, Ni, Cu, Zn, As, Pb, Cr, Fe, Co, Se and Cd. Some differences have been found in the organs studied, with especially relevant being the Cu-containing fraction present only in the brain and the As-containing one in the liver. Other differences related to the abundance of the metallospecies have also been found. The present paper is the first step in the study of the "metallome" of this inbred laboratory species from which the genome is completely known. This organism could be used as a model in future studies focused on wild mice and the analytical approach developed could be applied to wild mice to find markers of environmental pollution. [figure: see text] The present paper is the first step in the study of the "metallome" of the inbred laboratory specie Mus musculus from which the genome is completely known. Some interesting differences have been found in the extracts from the organs that are discussed along the text.

Published 24 December 2007 in Anal Bioanal Chem, 390(1): 17-28.
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