Chromatography Research Today is a free monthly online journal that collates and summarizes the latest research about Chromatography, including details on column chromatography, gas chromatography (gc), liquid chromatograpy, hplc.

A simpler analysis for the measurement of second virial coefficients by self-interaction chromatography.

Winzor DJ, Scott DJ, Wills PR

Department of Biochemistry, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Qld 4072, Australia. d.winzor@uq.edu.au

We describe a thermodynamic approach that supports the adoption of a simplified procedure for the determination of protein second virial coefficients (B(2)) by self-interaction chromatography. Its major advantage over the original method is a decrease in the number of parameters to which magnitudes must be assigned for the determination of B(2). Improved correlation of virial coefficients obtained by the chromatographic procedure with those obtained by light scattering is achieved by taking into account the twofold larger magnitudes of the former because of the experimental distinction between free and immobilized protein molecules in self-interaction chromatography.

Published 15 October 2007 in Anal Biochem, 371(1): 21-5.
Full-text of this article is available online (may require subscription).

© 2005-2009 Chromatography Research Today. All Rights Reserved.