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A hybrid FIA/HPLC system incorporating monolithic column chromatography.

Adcock JL, Francis PS, Agg KM, Marshall GD, Barnett NW

School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.

We have combined the generation of solvent gradients using milliGAT pumps, chromatographic separations with monolithic columns and chemiluminescence detection in an instrument manifold that approaches the automation and separation efficiency of HPLC, whilst maintaining the positive attributes of flow injection analysis (FIA), such as manifold versatility, speed of analysis and portability. As preliminary demonstrations of this hybrid FIA/HPLC system, we have determined six opiate alkaloids (morphine, pseudomorphine, codeine, oripavine, ethylmorphine and thebaine) and four biogenic amines (vanilmandelic acid, serotonin, 5-hydroxyindole-3-acetic acid and homovanillic acid) in human urine, using tris(2,2'-bipyridyl)ruthenium(III) and acidic potassium permanganate chemiluminescence detection.

Published 1 October 2007 in Anal Chim Acta, 600(1): 136-41.
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