Chromatography Research Today is a free monthly online journal that collates and summarizes the latest research about Chromatography, including details on column chromatography, gas chromatography (gc), liquid chromatograpy, hplc.

Simple determination of the HIV protease inhibitor atazanavir in human plasma by high-performance liquid chromatography with UV detection.

Loregian A, Pagni S, Ballarin E, Sinigalia E, Parisi SG, Palù G

Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padua 35121, Italy.

A simple high-performance liquid chromatography method for the determination of the human immunodeficiency virus protease inhibitor atazanavir in human plasma samples was developed and validated. The method involved a rapid and simple solid-phase extraction of atazanavir using Oasis HLB 1cc cartridges, an isocratic reversed-phase liquid chromatography on an XTerra RP18 (150mmx4.6mm, 3.5microm) column, and ultraviolet detection at 203nm. The mobile phase consisted of phosphate buffer (pH 6, 52.5mM) and acetonitrile (43:57, v/v). Up to 48 samples could be measured in one day since the run-time of one sample was 30min. The assay was linear from 0.04 to 10microg/ml with a lower limit of quantification of 0.04microg/ml. The mean absolute recovery of ATV was 98.1%. The method was precise, with both intra-day and inter-day coefficients of variation < or =3.0%, and accurate (deviations ranged from -3.0% to 4.5% and from -3.6% to 4.7% for intra-day and inter-day analysis, respectively). There was no interference from 35 tested potentially co-administrated drugs. This method provides a simple, sensitive, precise and reproducible assay for the therapeutic drug monitoring of atazanavir in clinical routine of laboratories with standard equipment.

Published 20 September 2006 in J Pharm Biomed Anal, 42(4): 500-5.
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