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A robust liquid chromatography tandem mass spectrometry method for total plasma homocysteine determination in clinical practice.

Ducros V, Belva-Besnet H, Casetta B, Favier A

Département de Biologie Intégrée, Centre Hospitalier Universitaire, Grenoble, France. vducros@chu-grenoble.fr

BACKGROUND: Total plasma homocysteine has emerged as an independent risk factor for vascular disease. To meet increasing requests by clinicians for this homocysteine determination, a rapid assay for a routine use has been developed. METHODS: A robust, stable, isotope-dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) method is described, including all the practical details and analytical performance results. RESULTS: The method allows homocysteine quantitation over a linear working range up to 100 micromol/L, with the limit of quantification estimated at a low value of 0.09 micromol/L. Total analytical imprecision is less than 4%. Accuracy was assessed by measuring the homocysteine concentration in a serum Standard Reference Material. CONCLUSIONS: The method was demonstrated to be quick, reliable and cheap after 1 year of use on a time-shared instrument in our hospital unit.

Published 1 August 2006 in Clin Chem Lab Med, 44(8): 987-90.
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