Chromatography Research Today is a free monthly online journal that collates and summarizes the latest research about Chromatography, including details on column chromatography, gas chromatography (gc), liquid chromatograpy, hplc.

Separation of hydrophobic solutes by organic-solvent-based micellar electrokinetic chromatography using cation surfactants.

Gong S, Liu F, Li W, Gao F, Gao C, Liao Y, Liu H

The Key Lab of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

In this study, the separation of 13 homologous stick-like hydrophobic solutes, i.e., biphenyl nitrile derivatives, by organic-solvent-based micellar electrokinetic chromatography (MEKC) was investigated in terms of separation medium composition, species and concentration of surfactant, other additives, separation voltage and temperature. The results showed that the 13 strong hydrophobic compounds were baseline separated in 25 min with a repeatability of less than 1.3% (RSD) for migration time. The separation medium was a mixture of methanol, 2-propanol and water (58.5:10:31.5), containing 150 mM cetyltrimethylammonium bromide (CTAB) and 20 mM sodium borate. Variety of solvent composition, temperature and applied voltage all showed remarkable effect on the separation. The organic-solvent-based MEKC method proved to be superior to the aqueous MEKC and microemulsion electrokinetic chromatography (MEEKC) methods for the separation of strongly hydrophobic compounds.

Published 3 July 2006 in J Chromatogr A, 1121(2): 274-9.
Full-text of this article is available online (may require subscription).

© 2005-2008 Chromatography Research Today. All Rights Reserved.