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Simple extraction method and gas chromatography-mass spectrometry in the selective ion monitoring mode for the determination of phenols in wine.

Minuti L, Pellegrino RM, Tesei I

Dipartimento di Chimica, Università degli Studi di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy. lucio@unipg.it

The concentrations of 22 components of wine, including most of those that have been shown to possess significant biological properties, have been determined by a fast and simple analytical method based on gas chromatography with mass spectrometric detection in the selective ion monitoring mode (GC/MS-SIM). The procedure involves an easy liquid-liquid extraction and derivatization methods of flavanols, phenols and carboxylic acid, using a very small wine volume. The average recovery (Rcv) ranged from 73 to 107% and linear regression coefficients (r(2)) were in a range of 0.981<or=r(2)>or=0.999. The GC/MS-SIM technique gives good specificity and sensitivity, and can therefore be suitable for routine monitoring of the concentration of individual phenolic antioxidants during winemaking and the aging process.

Published 1 May 2006 in J Chromatogr A, 1114(2): 263-8.
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