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Determination of polyunsaturated fatty acid monoepoxides by high performance liquid chromatography-mass spectrometry.

Fer M, Goulitquer S, Dréano Y, Berthou F, Corcos L, Amet Y

Laboratoire de Biochimie EA948, Faculté de Médecine, CS 93837, 29238-Brest-Cedex 3, France.

Despite the implication of polyunsaturated fatty acid monoepoxides in a large panel of biological effects, few methods allowing their separation in a single run are available. We describe here a simple method based on reversed-phase ion-pair high-performance liquid chromatography (RP-HPLC) and developed to successfully separate the various monoepoxides of eicosatrienoic, arachidonic, eicosapentaenoic and docosahexaenoic acids. These compounds were easily identified by liquid chromatography-mass spectrometry (LC-MS) with atmospheric pressure chemical ionisation owing to the volatility of counter-ion species. Compared to established methods, this new protocol proved its ability to totally resolve, in a single run, all of the different regioisomeric epoxides. In the long run, this method will demonstrate its efficacy to give insights into the cytochrome P450-dependent metabolism of polyunsaturated fatty acids (PUFAs) and the generation of physiologically active epoxy-derivatives.

Published 1 May 2006 in J Chromatogr A, 1115(1): 1-7.
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