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Screening for anabolic steroids in doping analysis by liquid chromatography/electrospray ion trap mass spectrometry.

Deventer K, Eenoo PV, Delbeke FT

Ghent University (UGent), Department of Clinical Chemistry, Microbiology and Immunology, Doping Control Laboratory (DoCoLab), Technologiepark 30, B-9052 Zwijnaarde, Belgium. Koen.Deventer@UGent.be

A fast and selective LC/MS/MS method for the screening of four anabolic steroids in human urine has been developed and validated. Liquid-liquid extraction with diethyl ether was applied after enzymatic hydrolysis. Analyses were performed on an ion trap mass spectrometer equipped with electrospray ionisation. MS/MS was applied for all compounds. The analytical run time was 11 min. The LOD for all compounds varied between 1 and 10 ng/mL. Left-over A samples, which were declared positive by GC/MS for the presence of 3'-hydroxystanozolol, were assessed using the described method.

Published 1 May 2006 in Biomed Chromatogr, 20(5): 429-33.
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