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Determination of five coumarins in Cnidii Fructus by micellar electrokinetic capillary chromatography.

Wu MH, Zhao LH, Song Y, Zhang W, Xiang BR, Mei LH

Key Laboratory of Modern Chinese Medicine, Ministry of Education, Nanjing, P.R. China.

A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.

Published 5 January 2006 in Planta Med, 71(12): 1152-6.
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