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Development of a solid phase microextraction-gas chromatography method to determine N-hydroxymethyl-N-methylformamide and N-methylformamide in urine.

Knupp VF, Leite EM, de Lourdes Cardeal Z

Chemistry Department, ICEx, Federal University of Minas Gerais, Av. Antonio Carlos 6627, C.P. 702, 31270901 Belo Horizonte, M.G., Brazil.

A headspace solid phase microextraction (SPME) method has been developed to determine metabolites of dimethylformamide, N-hydroxymethyl-N-methylformamide, and N-methylformamide (NMF) as NMF in urine by gas chromatography with nitrogen-phosphorus detector (GC-NPD). An SPME holder with a 65-microm PDMS/DVB fiber coating was used. Optimal desorption conditions were 250 degrees C for 1 min, adsorption at 80 degrees C for 15 min, and 3.00 mL of sample in the headspace vial. The method presented good resolution, repeatability, recovery, detection limit, ruggedness and response linearity.

Published 5 December 2005 in J Chromatogr B Analyt Technol Biomed Life Sci, 828(1): 103-7.
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