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Selective photo-reduction of N-nitroamines combined with micellar electrokinetic chromatography and laser fluorimetric detection.

Bell LM, Murray GM

Hewlett Packard Company, 1000 NE Circle Blvd., Corvallis, OR 97330, USA. leann.bell@hp.com

N-nitroso compounds (NOC) are potent carcinogens. Reliable methods for the analysis of volatile carcinogenic NOC are well established; however selective and sensitive methods for routine analysis of thermally unstable, ionic or non-volatile NOC are still needed. For this purpose, a method based on micellar electrokinetic chromatography (MEKC) with laser induced fluorescence (LIF) detection is described for the simultaneous determination of a broad range of N-nitroso compounds. In this procedure, the nitroso group is photolytically cleaved from the NOC to yield the corresponding amine. The amines are then derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl), identified and quantified using MEKC-LIF. For the standard mixture of NOC, this method has good sensitivity and a large dynamic range. The detection limit provided by the method is 9 ppb for N-nitrosopyrrolidine.

Published 17 October 2005 in J Chromatogr B Analyt Technol Biomed Life Sci, 826(1): 160-8.
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